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Haplogroup H Probably Originated in Africa
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[QUOTE]Originally posted by Troll Patrol # Ish Gebor: [QB] ^You still don't get it, you still keep citing frequencies. When it's not solely about frequencies. Iteration is yo' thang'. http://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&p=PMC3&id=3978205_emss-57674-f0001.jpg Mitochondrial haplogroup H sequence evolution (a) Phylogenetic network of 39 prehistoric mitochondrial genomes sorted into two temporal groupings: Early Neolithic (left) and Mid-to-Late Neolithic (right). Node colours represent archaeological cultures. (b) A Bayesian skyride plot of 200 representative present-day and 39 ancient hg H mt genomes (the thick red line denotes the posterior median, thinner flanking lines denote the 95% credibility interval; note the logarithmic scale of the y-axis). Prehistoric samples (18 radiocarbon and 21 mean archaeological dates) served as internal calibration points (black bars). For comparison, census size estimates for the European population are shown as orange dots. Population density estimates from the archaeological record for key periods in Central Europe are plotted as blue squares in chronological order: LBK, Iron Age, Roman period, Merovingian, and Pre-industrial modern times (y-axis on the right)28. http://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&p=PMC3&id=3978205_emss-57674-f0002.jpg Population affinities of select Neolithic cultures (a) PCA biplot based on the frequencies of 15 hg H sub-haplogroups (component loadings) from 37 present-day Western Eurasian and three ancient populations (light blue: Western Europe; dark blue: Central and Eastern Europe; orange; Near East, Caucasus, and Anatolia; and pink: ancient samples). Populations are abbreviated as follows: GAL, Galicia; CNT, Cantabria; CAT, Catalonia; GAS, Galicia/Asturia; CAN, Cantabria2; POT, Potes; PAS, Pasiegos; VIZ, Vizcaya; GUI, Guipuzcoa; BMI, Basques; IPNE, Iberian Peninsula Northeast; TUR, Turkey; ARM, Armenia; GEO, Georgia; NWC, Northwest Caucasus; DAG, Dagestan; OSS, Ossetia; SYR, Syria; LBN, Lebanon; JOR, Jordan; ARB, Arabian Peninsula; ARE, Arabian Peninsula2; KBK, Karachay-Balkaria; MKD, Macedonia; VUR, Volga-Ural region; FIN, Finland; EST, Estonia; ESV, Eastern Slavs; SVK, Slovakia; FRA, France; BLK, Balkans; DEU, Germany; AUT, Austria, ROU, Romania; FRM, France Normandy; WIS, Western Isles; CZE, Czech Republic; LBK, Linear pottery culture; BBC, Bell Beaker culture; MNE, Middle Neolithic. (b) Procrustes analyses of geographic coordinates and PCA scores of the same dataset (similarity score t0 = 0.733, p ... 10−6, 100 000 permutations). (c) Ward clustering dendrogram of the three ancient groups and present-day populations (colour code as above and p values in % of approximately unbiased bootstrapping for the following three main clusters). (d) Results of the model-based test to identify the number of clusters by the model with the highest support (highest BIC; VEV= multivariate mixture model (ellipsoidal, equal shape)). http://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&p=PMC3&id=3978205_emss-57674-f0003.jpg Schematic representation of experimental steps (a) Probe DNA was prepared by amplifying a complete mitochondrial genome in two overlapping fragments by long-range PCR, followed by DNA fragmentation and biotinylation to form mtDNA ‘baits’ for targeted hybridisation. (b) Ancient DNA was enzymatically blunt-ended and phosphorylated, ligated to custom library adapters, followed by polymerase ‘fill-in’ to create ‘immortalised’ double-stranded DNA libraries. (c) Hybridisation-based DNA-capture using biotinylated probe bound to Streptavidin magnetic beads; following stringency washes, captured library constructs enriched in mtDNA sequences are eluted from the beads/probe via a novel polymerase strand-displacement reaction followed by PCR library reamplification. These steps can be carried out iteratively to maximise mtDNA content in enriched libraries [/QB][/QUOTE]
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